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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vmireaviz</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник медицинского института «РЕАВИЗ». Реабилитация, Врач и Здоровье</journal-title><trans-title-group xml:lang="en"><trans-title>Bulletin of the Medical Institute "REAVIZ" (REHABILITATION, DOCTOR AND HEALTH)</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2226-762X</issn><issn pub-type="epub">2782-1579</issn><publisher><publisher-name>РЕАВИЗ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.20340/vmi-rvz.2025.5.PROT.1</article-id><article-id custom-type="elpub" pub-id-type="custom">vmireaviz-1405</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Клинические протоколы</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Clinical Protocols</subject></subj-group></article-categories><title-group><article-title>Клинический протокол гипотермической оксигенированной перфузии (HOPE) и контролируемой оксигенированной реваскуляризации (COR) печени и почек</article-title><trans-title-group xml:lang="en"><trans-title>Clinical Protocol for Hypothermic Oxygenated Perfusion (HOPE) and Controlled Oxygenated Rewarming (COR) of Liver and Kidneys</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6362-7914</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Новрузбеков</surname><given-names>М. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Novruzbekov</surname><given-names>M. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Новрузбеков Мурад Сафтарович, д-р мед. наук, профессор, врач-хирург, руководитель научного отделения, Большая Сухаревская пл., д. 3, г. Москва, 129090;</p><p>Каширское шоссе, д. 23, г. Москва, 115522;</p><p>заведующий кафедрой трансплантологии и искусственных органов им. В.П. Демихова, ул. Островитянова, д. 1, г. Москва, 117513;</p><p>профессор кафедры хирургических болезней, Краснобогатырская ул., д. 2, стр. 2, Москва, 107564</p></bio><bio xml:lang="en"><p>Murad S. Novruzbekov, Dr. Sci. (Med.), Professor, Surgeon, Head of the Research Department, Bolshaya Sukharevskaya Square, 3, Moscow, 129090;</p><p>Kashirskoe shosse, 23, Moscow, 115522;</p><p> Head of the Department of Transplantology and Artificial Organs named after V.P. Demikhov, Ostrovityanova St., 1, Moscow, 117513;</p><p>Professor, Department of Surgical Diseases, Krasnobogatyrskaya str., 2, building 2, Moscow, 107564</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5889-8675</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Яремин</surname><given-names>Б. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Yaremin</surname><given-names>B. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Яремин Борис Иванович, канд. мед. наук, врач-хирург, научный сотрудник, Большая Сухаревская пл., д. 3, г. Москва, 129090;</p><p>Каширское шоссе, д. 23, г. Москва, 115522;</p><p>доцент кафедры трансплантологии и искусственных органов им. В.П. Демихова, ул. Островитянова, д. 1, г. Москва, 117513;</p><p>заведующий кафедрой хирургических болезней, Краснобогатырская ул., д. 2, стр. 2, Москва, 107564</p></bio><bio xml:lang="en"><p>Boris I. Yaremin, Cand. Sci. (Med.), surgeon, researcher, Bolshaya Sukharevskaya Square, 3, Moscow, 129090;</p><p>Kashirskoe shosse, 23, Moscow, 115522;</p><p>Associate Professor, V.P. Demikhov Department of Transplantology and Artificial Organs, Ostrovityanova St., 1, Moscow, 117513;</p><p>Head of the Department of Surgical Diseases, Krasnobogatyrskaya str., 2, building 2, Moscow, 107564</p></bio><email xlink:type="simple">b.i.yaremin@reaviz.online</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1396-7048</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Балкаров</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Balkarov</surname><given-names>A. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Балкаров Аслан Галиевич, канд. мед. наук, руководитель научного отделения, Большая Сухаревская пл., д. 3, г. Москва, 129090;</p><p>доцент кафедры трансплантологии и искусственных органов им. В.П. Демихова, ул. Островитянова, д. 1, г. Москва, 117513</p></bio><bio xml:lang="en"><p>Aslan G. Balkarov, Cand. Sci. (Med.), Head of the Research Department, Bolshaya Sukharevskaya Square, 3, Moscow, 129090;</p><p>Associate Professor, V.P. Demikhov Department of Transplantology and Artificial Organs, Ostrovityanova St., 1, Moscow, 117513</p></bio><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5723-4818</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Казымов</surname><given-names>Б. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Kazymov</surname><given-names>B. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Казымов Бахтияр Исмет Оглы, врач-хирург, научный сотрудник, Большая Сухаревская пл., д. 3, г. Москва, 129090;</p><p>Каширское шоссе, д. 23, г. Москва, 115522;</p><p>ассистент кафедры хирургических болезней, Краснобогатырская ул., д. 2, стр. 2, Москва, 107564</p></bio><bio xml:lang="en"><p>Bakhtiyar I. Kazymov, Surgeon, Researcher, Bolshaya Sukharevskaya Square, 3, Moscow, 129090;</p><p>Kashirskoe shosse, 23, Moscow, 115522;</p><p>Assistant Professor, Department of Surgical Diseases, Krasnobogatyrskaya str., 2, building 2, Moscow, 107564</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0241-1298</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Аносова</surname><given-names>Е. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Anosova</surname><given-names>E. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Аносова Екатерина Юрьевна, врач-хирург, ассистент кафедры оперативной хирургии и топографической анатомии, </p><p>ул. Островитянова, д. 1, г. Москва, 117513</p></bio><bio xml:lang="en"><p>Ekaterina Yu. Anosova, Surgeon, Assistant Professor, Department of Operative Surgery and Topographic Anatomy,</p><p>Ostrovityanova St., 1, Moscow, 117513</p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3799-7698</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Свищева</surname><given-names>П. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Svishcheva</surname><given-names>P. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Свищева Полина Олеговна, врач-патологоанатом, </p><p>Большая Сухаревская пл., д. 3, г. Москва, 129090</p></bio><bio xml:lang="en"><p>Polina O. Svischeva, Pathologist,</p><p>Bolshaya Sukharevskaya Square, 3, Moscow, 129090</p></bio><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8055-1958</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Павлова</surname><given-names>О. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Pavlova</surname><given-names>O. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Павлова Ольга Николаевна, д-р биол. наук, заведующая кафедрой физиологии, ул. Чапаевская, д. 89, г. Самара, 443099;</p><p>профессор кафедры морфологии и патологии, ул. Чапаевская, д. 227, г. Самара, 443001</p></bio><bio xml:lang="en"><p>Ol'ga N. Pavlova, Dr. Sci. (Biol.), Head of the Department of Physiology, Chapaevskaya St., 89, Samara, 443099;</p><p>Professor of the Department of Morphology and Pathology, Chapaevskaya St., 227, Samara, 443001</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-0057-9326</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ханова</surname><given-names>С. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Khanova</surname><given-names>S. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ханова Сафия Магомедовна, врач-клинический ординатор, </p><p>Большая Сухаревская пл., д. 3, г. Москва, 129090</p></bio><bio xml:lang="en"><p>Safiya M. Khanova, Clinical Resident, </p><p>Bolshaya Sukharevskaya Square, 3, Moscow, 129090</p></bio><xref ref-type="aff" rid="aff-5"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Научно-исследовательский институт скорой помощи им. Н.В. Склифосовского;&#13;
Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина;&#13;
Российский национальный исследовательский медицинский университет им. Н.И. Пирогова;&#13;
Московский медицинский университет «Реавиз»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Sklifosovsky Research Institute for Emergency Medicine;&#13;
National Medical Research Center of Oncology named after N.N. Blokhin;&#13;
Pirogov Russian National Research Medical University;&#13;
Moscow Medical University "Reaviz"</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Научно-исследовательский институт скорой помощи им. Н.В. Склифосовского;&#13;
Российский национальный исследовательский медицинский университет им. Н.И. Пирогова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Sklifosovsky Research Institute for Emergency Medicine;&#13;
Pirogov Russian National Research Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Научно-исследовательский институт скорой помощи им. Н.В. Склифосовского;&#13;
Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина;&#13;
Московский медицинский университет «Реавиз»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Sklifosovsky Research Institute for Emergency Medicine;&#13;
National Medical Research Center of Oncology named after N.N. Blokhin;&#13;
Moscow Medical University "Reaviz"</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Российский национальный исследовательский медицинский университет им. Н.И. Пирогова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Pirogov Russian National Research Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru"><institution>Научно-исследовательский институт скорой помощи им. Н.В. Склифосовского</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Sklifosovsky Research Institute for Emergency Medicine</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-6"><aff xml:lang="ru"><institution>Самарский государственный медицинский университет;&#13;
Медицинский университет «Реавиз»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Samara State Medical University;&#13;
Medical University "Reaviz"</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>27</day><month>01</month><year>2026</year></pub-date><volume>15</volume><issue>5</issue><fpage>202</fpage><lpage>223</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Новрузбеков М.С., Яремин Б.И., Балкаров А.Г., Казымов Б.И., Аносова Е.Ю., Свищева П.О., Павлова О.Н., Ханова С.М., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Новрузбеков М.С., Яремин Б.И., Балкаров А.Г., Казымов Б.И., Аносова Е.Ю., Свищева П.О., Павлова О.Н., Ханова С.М.</copyright-holder><copyright-holder xml:lang="en">Novruzbekov M.S., Yaremin B.I., Balkarov A.G., Kazymov B.I., Anosova E.Y., Svishcheva P.O., Pavlova O.N., Khanova S.M.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestnik.reaviz.ru/jour/article/view/1405">https://vestnik.reaviz.ru/jour/article/view/1405</self-uri><abstract><p>Клинический протокол описывает применение гипотермической оксигенированной перфузии (HOPE) и контролируемой оксигенированной реваскуляризации (COR) для улучшения качества донорских органов печени и почек. Протокол основан на международных и отечественных исследованиях, демонстрирующих снижение частоты билиарных осложнений и ранней дисфункции трансплантата при использовании машинной перфузии. HOPE рекомендуется для органов от доноров с асистолией и расширенными критериями, в то время как COR применяется для оценки маргинальных органов. Протокол включает детальное описание показаний, противопоказаний, технических аспектов перфузии, мониторинга и оценки жизнеспособности органов с использованием биомаркеров, таких как флавин мононуклеотид, сукцинат, маркеры апоптоза, некроптоза и ферроптоза. Представлены собственные результаты авторов, подтверждающие эффективность методов в снижении осложнений и улучшении исходов трансплантации.</p></abstract><trans-abstract xml:lang="en"><p>This clinical protocol outlines the application of Hypothermic Oxygenated Perfusion (HOPE) and Controlled Oxygenated Revascularization (COR) to enhance the quality of donor liver and kidney grafts. The protocol is based on international and domestic studies demonstrating reduced biliary complications and early graft dysfunction rates with machine perfusion. HOPE is recommended for organs from donors after circulatory death (DCD) and extended criteria donors (ECD), while COR is used for assessing marginal organs. The protocol includes detailed descriptions of indications, contraindications, technical aspects of perfusion, monitoring, and viability assessment using biomarkers such as flavin mononucleotide, succinate, and markers of apoptosis, necroptosis, and ferroptosis. The authors present their own results, confirming the effectiveness of these methods in reducing complications and improving transplantation outcomes.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>трансплантация печени [D016031]</kwd><kwd>макростеатоз [D005234]</kwd><kwd>машинная перфузия [D010477]</kwd><kwd>гипотермическая оксигенированная перфузия [D010477]</kwd><kwd>ишемически-реперфузионное повреждение [D015427]</kwd><kwd>консервация органов [D009928]</kwd><kwd>окислительный стресс [D018384]</kwd><kwd>митохондриальная дисфункция [D028361]</kwd><kwd>апоптоз [D017209]</kwd><kwd>ферроптоз [D000081946]</kwd><kwd>маргинальные доноры [D014019 (подкатегория)]</kwd><kwd>холодовая ишемия [D007511]</kwd><kwd>липидная пероксидация [D015227]</kwd><kwd>глутатион [D005978]</kwd><kwd>каспазы [D020170]</kwd><kwd>цитокины [D016207]</kwd><kwd>трансаминазы [D000410]</kwd><kwd>выживаемость трансплантата [D006085]</kwd></kwd-group><kwd-group xml:lang="en"><kwd>liver transplantation [D016031]</kwd><kwd>macrosteatosis [D005234]</kwd><kwd>machine perfusion [D010477]</kwd><kwd>hypothermic oxygenated perfusion [D010477]</kwd><kwd>ischemia-reperfusion injury [D015427]</kwd><kwd>organ preservation [D009928]</kwd><kwd>oxidative stress [D018384]</kwd><kwd>mitochondrial dysfunction [D028361]</kwd><kwd>apoptosis [D017209]</kwd><kwd>ferroptosis [D000081946]</kwd><kwd>marginal donors [D014019 (subcategory)]</kwd><kwd>cold ischemia [D007511]</kwd><kwd>lipid peroxidation [D015227]</kwd><kwd>glutathione [D005978]</kwd><kwd>caspases [D020170]</kwd><kwd>cytokines [D016207]</kwd><kwd>transaminases [D000410]</kwd><kwd>graft survival [D006085]</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Schlegel A, Muller X, Kalisvaart M, et al. 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