The state of oxidative processes in testicular tissue samples after ionizing radiation
https://doi.org/10.20340/vmi-rvz.2024.6.MORPH.3
Abstract
Aim. To study of oxidative processes in testicular tissues after gamma irradiation.
Material and methods. Using the polarographic method, the rate of oxygen absorption on endogenous and exogenous substrates (Vend, Vsuc, Vglu, Vdnf) and with specific inhibitors of tissue respiration of amytal and sodium malonate (Vam, Vmal) in testicular tissues at different times (3, 10, 40 days) after total single gamma irradiation (1.0 Gr).
Results. It was found that, in the testicular tissues of rats at different times (3rd, 10th, 40th day) after total single gamma irradiation (1.0 Gy). In the early period (72 hours) after irradiation, (Vend) decreased by 14.7 % (p < 0.05), (Vsuc) by 18.6 % (p < 0.05) and (Vglu) by 10.9 % (p < 0.05) compared with the control. After 10 days, on the contrary, there was an increase in (Vend) by 108.2 % (p < 0.05), in the presence of exogenous succinate and glutamate substrates, increased (Vsuc) by 45.1 % (p < 0.05) and (Vglu) by 112 % (p < 0.05), SDdnf decreased by 12.8 % (p < 0.05), and with inhibitors, low-resistance respiration (MRD) increased by 9.4 % (p < 0.05). On day 40 (Vend, Vsuc, Vglu) increased by 120.7 % (p < 0.05), 124.8 % (p < 0.05) and 97.1 % (p < 0.05), respectively, SDdnf decreased by 11.5 % (p < 0.001), amytal resistant respiration (ARD) by 30.6 % (p < 0.05) and MRD by 11.7 % (p < 0.05).
Conclusion. Increased oxygen uptake was accompanied by a significant decrease in SDdnf in both experimental groups (10th and 40th days), which suggested the threat of disconnection of oxidation and phosphorylation processes. A decrease in MPD also indicates a decrease in the contribution of fatty acids (FA) to the energy supply of testicular tissue.
About the Author
M. A. Al Meselmani
Polessky State University
Belarus
Competing Interests:
Belarus
Mohanad Ali Al Meselmani, Cand. Sci. (Biol.), Associate Professor of the Department of Biochemistry and Bioinformatics
Author ID: 1200106
23, Dneprovskoy Flotiliya st., Pinsk, 225710
Competing Interests:
The authors declare no competing interests
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Review
For citations:
Al Meselmani M.A. The state of oxidative processes in testicular tissue samples after ionizing radiation. Bulletin of the Medical Institute "REAVIZ" (REHABILITATION, DOCTOR AND HEALTH). 2024;14(6):17-23. (In Russ.) https://doi.org/10.20340/vmi-rvz.2024.6.MORPH.3
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