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Modern spleen plastination technologies: comparative analysis of techniques and materials (literature review)

https://doi.org/10.20340/vmi-rvz.2025.3.MORPH.1

Abstract

The use of cadaveric material has found its application for educational purposes in veterinary and medical universities. The obvious disadvantages of this approach are the requirements for maintaining the operational capacity of the cadaver material, as well as the peculiarities of changing the morphological structure of the organ with some cooking technologies. The article examines the application of modern methods of spleen plastination based on a sample from scientific data from PubMed, eLibrary and Google Scholar in the period from 1994 to 2024. 21 articles from a sample of 252 articles were analyzed. Currently, the authors propose standard Hagens plastination techniques with Biodur S10; using various fixing solutions (in particular, 0.56% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer (pH 7.4) containing 1.2 mM MgCl2 and 2.5 mM CaCl2); with Biodur S10/S15; with Brazilian polysiloxane P1; сryodehydration methods, room-temperature plastination technique, as well as methods of own production.

About the Authors

M. A. Kislov
Pirogov Russian National Research Medical University
Russian Federation

Maksim A. Kislov - Dr. Sci. (Med.), Docent, Head of the Department of Morphology at the Institute of Anatomy and Morphology 
Author's contribution: study concept and design.

1, Ostrovityanova St., Moscow, 117513


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



K. N. Krupin
Pirogov Russian National Research Medical University
Russian Federation

Konstantin N. Krupin - Cand. Sci. (Med.), Docent, specialist in educational and methodological work of the Center for Anatomical and Simulation Modeling of the Multi-profile Accreditation and Simulation Center of the Institute of Anatomy and Morphology named after Academician Y.M. Lopukhin 
Аuthor's contribution: literature data analysis.

1, Ostrovityanova St., Moscow, 117513


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



A. A. Supil'nikov
Pirogov Russian National Research Medical University; Medical University Reaviz
Russian Federation

Aleksey A. Supilnikov - Cand. Sci. (Med.), Docent, Deputy Director of the Director of the Lopukhin Institute of Anatomy and Morphology; First Vicerector for Scientific Activity 
Аuthor's contribution: literature data analysis.

1, Ostrovityanova St., Moscow, 117513;
227, Chapaevskaya St., Samara, 443001


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



N. V. Ignatkin
Pirogov Russian National Research Medical University
Russian Federation

Nikita V. Ignatkin - Student 
Author's contribution: selection of literary sources, writing the text of the article.

1, Ostrovityanova St., Moscow, 117513


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



A. Yu. Kirillov
Samara Regional Bureau of Forensic Medicine
Russian Federation

Aleksandr Yu. Kirillov - Forensic medical expert, head of the Togliatti forensic medical department 
Author's contribution: selection of literary sources, writing the text of the article.

51, Tukhachevsky St., Samara, 443082


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



T. V. Pistsova
Pirogov Russian National Research Medical University
Russian Federation

Tat'yana V. Pistsova - Candidate of Medical Sciences, Associate Professor, Professor of the Department of Morphology 
Author's contribution: selection of literary sources, writing the text of the article.

1, Ostrovityanova St., Moscow, 117513


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



A. E. Farkhadova
Patrice Lumumba Peoples' Friendship University of Russia
Russian Federation

Al'bina E. Farkhadova - 6th year student of the Faculty of Medicine 
Author's contribution: work with sources, analysis and generalization of the obtained data.

6, Miklukho-Maklaya st., Moscow, 117198


Competing Interests:

The authors declare no competing interests. Supilnikov A.A. is a member of the editorial board of the journal, did not participate in the decision to publish the article



References

1. Grondin G, Grondin G.G, Talbot BG. A study of criteria permitting the use of plastinated specimens for light and electron microscopy. Biotech Histochem. 1994 Jul;69(4):219-34. doi: 10.3109/10520299409106291.

2. Yunus HA, Ekim O, Bakıcı C, Batur B, Çakır A. From toxic cadavers to biosafe specimens: a brief history of plastination in veterinary anatomy. Vet Hekim Der Derg. 2022;93(2):158-65. https://doi.org/10.33188/vetheder.998978

3. Monteiro, Y. F., Silva, M. V. F., Bittencourt, A. P. S. V., & Bittencourt, A. S. (2022). Plastination with low viscosity silicone: strategy for less tissue shrinkage. Brazilian Journal of Medical and Biological Research, 55, e11962. http://doi: 10.1590/1414-431X2022e11962

4. Pendovski, L., Bozhinovski, D., Ilievska, K., Trojachanec, P., & Ilieski, V. (2022). Computer tomography (CT) scans as a diagnostic tool for interpretation of S10 plastinated slides from dog cadaver. Macedonian Veterinary Review, 45(2), 127-135. https://doi.org/10.2478/macvetrev-2022-0020

5. Boduç, E. (2022). Plastination with all its features: immortalization of the corpse. Advances in medicine and health science, 119.

6. Islam, R., Ayman, U., & Sultana, N. (2021). Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat. International Journal of Morphology, 39(3). http://dx.doi.org/10.4067/S0717-95022021000300759

7. Sultana, N., & Islam, R. (2023). Efficacy of cryodehydration technique in preserving the gross and histoarchitectural details of goat visceral and musculoskeletal specimens. Journal of Advanced Veterinary and Animal Research, 10(4), 720. http://doi: 10.5455/javar.2023.j727


Review

For citations:


Kislov M.A., Krupin K.N., Supil'nikov A.A., Ignatkin N.V., Kirillov A.Yu., Pistsova T.V., Farkhadova A.E. Modern spleen plastination technologies: comparative analysis of techniques and materials (literature review). Bulletin of the Medical Institute "REAVIZ" (REHABILITATION, DOCTOR AND HEALTH). 2025;15(3):145-150. (In Russ.) https://doi.org/10.20340/vmi-rvz.2025.3.MORPH.1

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ISSN 2226-762X (Print)
ISSN 2782-1579 (Online)