Modern spleen plastination technologies: comparative analysis of techniques and materials (literature review)

The use of cadaveric material has found its application for educational purposes in veterinary and medical universities. The obvious disadvantages of this approach are the requirements for maintaining the operational capacity of the cadaver material, as well as the peculiarities of changing the morphological structure of the organ with some cooking technologies. The article examines the application of modern methods of spleen plastination based on a sample from scientific data from PubMed, eLibrary and Google Scholar in the period from 1994 to 2024. 21 articles from a sample of 252 articles were analyzed. Currently, the authors propose standard Hagens plastination techniques with Biodur S10; using various fixing solutions (in particular, 0.56% glutaraldehyde and 2% formaldehyde in 0.1 M phosphate buffer (pH 7.4) containing 1.2 mM MgCl₂ and 2.5 mM CaCl₂); with Biodur S10/S15; with Brazilian polysiloxane P1; сryodehydration methods, room-temperature plastination technique, as well as methods of own production.

1. Grondin G, Grondin G.G, Talbot BG. A study of criteria permitting the use of plastinated specimens for light and electron microscopy. Biotech Histochem. 1994 Jul;69(4):219-34. https://doi.org/10.3109/10520299409106291

2. Yunus HA, Ekim O, Bakıcı C, Batur B, Çakır A. From toxic cadavers to biosafe specimens: a brief history of plastination in veterinary anatomy. Vet Hekim Der Derg. 2022;93(2):158-65. https://doi.org/10.33188/vetheder.998978

3. De Jong K, Henry RW. Silicone plastination of biological tissue: cold-temperature technique Biodur S10/S15 technique and products. J Int Soc Plastinat. 2007;22:2-14.

4. Martinez HR, Ottone NE. Plastination of Old Formalin-Fixed Specimens. J Plastination. 2011;23:8-14.

5. Raoof A. Silicone Plastination of Biological Tissue: Room-temperature Technique Dow™/Corcoran Technique and Products. J Plastination. 2001;16:19-25.

6. Mohamed AMA. Establishing for the First Time the Use of the Standard S10 Technique for Plastination in The Sudan. J Plastination. 2018;30(2):16-21.

7. Pendovski L., Bozhinovski D., Ilievska K., Trojachanec P., Ilieski V. Computer tomography (CT) scans as a diagnostic tool for interpretation of S10 plastinated slides from dog cadaver. Macedonian Veterinary Review. 2022;45(2):127-135. https://doi.org/10.2478/macvetrev-2022-0020

8. Cerqueira EP, Baptista CAC, Campi CC, Silva AF. 3D Reconstruction of Silicone (S10 Biodur®) Plastinated Specimens Using Computed Tomography Scanning. J Plastination. 2011;23:19-28.

9. Boduç, E. Plastination with all its features: immortalization of the corpse. Advances in medicine and health science. 2022;119.

10. Sagane Y, Kido H, Nakazawa Y, Nakajima Y, Nakajima Y, Takahashi M, Niikura K, Mazaki H, Fukushi H, Muramatsu K, Kodama M. Analysis of commercial plastination agents. Surg Radiol Anat. 2004;26(1):29-33. https://doi.org/10.1007/s00276-003-0216-9

11. Monteiro Y.F., Silva M.V.F., Bittencourt A.P.S.V., Bittencourt A.S. Plastination with low viscosity silicone: strategy for less tissue shrinkage. Brazilian Journal of Medical and Biological Research. 2022;55:e11962. https://doi.org/10.1590/1414-431X2022e11962

12. Monteiro YF, Silva MVF, Bittencourt APSV, Bittencourt AS. Plastination with low viscosity silicone: strategy for less tissue shrinkage. Braz J Med Biol Res. 2022 Jul 13;55. https://doi.org/10.1590/1414-431X2022e11962

13. Delpupo FVB, Cassiano LG, Monteiro YF, Júnior MC, Soares K, Bittencourt AS. Low viscosity silicone with less shrinkage for brain slices. Anat Histol Embryol. 2021;50(1):156-167. https://doi.org/10.1111/ahe.12627

14. Islam R., Ayman U., Sultana N. Histological Architectures and Biometric Characteristics of Indigenously Plastinated Organs of Goat. International Journal of Morphology. 2021;39(3). http://dx.doi.org/10.4067/S0717-95022021000300759

15. Sultana N., Islam R. Efficacy of cryodehydration technique in preserving the gross and histoarchitectural details of goat visceral and musculoskeletal specimens. Journal of Advanced Veterinary and Animal Research. 2023;10(4):720. https://doi.org/10.5455/javar.2023.j727

16. Starchik D.A. Epoxy plastination. International Morphological Center. (In Russ.). URL: http://www.anatomia.ru/about/technology/plastinaciya_raspi/

17. Plastination. Wikipedia. (In Russ.). URL: https://ru.wikipedia.org/wiki/Пластинация

18. Starchik D.A. Plastination. International Morphological Center. (In Russ.). URL: http://www.anatomia.ru/about/plastination/

19. Smodlaka H, Henry RW. Silicone Plastination of Biological Tissue: Cold-temperature Technique North Carolina Technique and Products. J Plastination. 2003;18:25-29.

20. Glover RA, Henry RW, Wade RS. Polymer preservation technology: polyester plastination for museum collections and teaching specimens. J Int Soc Plastination. 1998;13(2):39.

21. Cyprianj. THE SCIENCE OF PLASTINATION- A way of preserving tissues for centuries using a curable polymer (Biodur S10). Steemit Internet. 2018 Jun 22. URL: https://steemit.com/steemstem/@cyprianj/the-science-of-plastination-a-way-of-preserving-tissues-for-centuries-using-acurable-polymer-biodur-s10