Primary results of replacement the experimental hyaline cartilage defect with human fibroblast cell culture
M. S. Bozhokin,
D. M. Marchenko,
E. R. Mikhailova,
A. P. Antipov,
Yu. A. Nashchekina,
D. B. Vcherashnij,
S. V. Novoseltsev,
V. N. Kruglov https://doi.org/10.20340/vmi-rvz.2022.2.MORPH.1
Abstract
The hyaline cartilage damage problem is actual, but existing recovery methods are not fully sufficient. A promising solution of this problem can be the implantation of a tissue-engineered structure (TEC), consisting of a cell culture and a biodegradable scaffold. In this experiment we created a construct consisting of a polylactide scaffold and a human dermal fibroblasts culture. After implantation of TEC in the modeled defect area we analyzed the experimental group regeneration results in comparison to the control group.
About the Authors
M. S. Bozhokin
National Medical Research Center traumatology and orthopedics named after R.R. Vreden;
Institute of Cytology Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
D. M. Marchenko
Saint Petersburg State University
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
E. R. Mikhailova
Institute of Cytology Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
A. P. Antipov
National Medical Research Center traumatology and orthopedics named after R.R. Vreden
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
Yu. A. Nashchekina
Institute of Cytology Russian Academy of Sciences
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
D. B. Vcherashnij
Ioffe Physical Technical Institute
Russian Federation
Competing Interests:
Russian Federation
Saint Petersburg
Competing Interests:
The authors declare no competing interests
S. V. Novoseltsev
I.M. Sechenov First Moscow State Medical University
Russian Federation
Competing Interests:
Russian Federation
Moscow
Competing Interests:
The authors declare no competing interests
V. N. Kruglov
Reaviz Medical University
Russian Federation
Competing Interests:
Russian Federation
Samara
Competing Interests:
The authors declare no competing interests
References
1. Widuchowski W., Widuchowski J., Trzaska T. Articular cartilage defects: study of 25,124 knee arthroscopies. Knee. 2007;14(3):177-182.
2. Kurtz, S., Ong, K., Lau, E., Mowat, F., and Halpern, M. (2007) Projections of primary and revision hip and knee arthroplasty in the United States from 2005 to 2030, J. Bone Jt. Surg. – Ser. A., 89, 780785. https://doi.org/10.2106/JBJS.F.00222
3. Bozhokin M.S., Bozhkova S.A., Netylko G.I., Rumakin V.P., Nakonechny D.G., Chepurnenko M.N. (2017) Morphofunctional characteristics of the chondroregeneration process in an experimental local defect of the articular cartilage surface. International Journal of Applied and Basic Research. 2017;8:302-306. (In Russ).
4. Maglio, M., Brogini, S., Pagani, S., Giavaresi, G., and Tschon, M. Current trends in the evaluation of osteochondral lesion treatments: histology, histomorphometry, and biomechanics in preclinical models. Bio. Med. Research Intern. 2019:4040236.
5. Bozhokin M. S., Bozhkova S. A., Netylko G. I. Possibilities of modern cellular technologies for the restoration of damaged articular cartilage (analytical review of the literature). Traumatology and orthopedics of Russia. 2016;22:122-134. (In Russ).
6. Jeuken R., Roth A., Peters R., Van Donkelaar C., Thies J., Van Rhijn L., and Emans P. J. Polymers in cartilage defect repair of the knee: current status and future prospects. Polymers. 2016;8(6):219.
7. Utility model patent No. 190863 U1 Russian Federation, IPC A61M 1/00. Device for combining cell culture and biodegradable carrier: No. 2019104311: Appl. 02/15/2019: publ. 07/15/2019 / M.S. Bozhokin, S.A. Bozhkova, E.M. Litvinov, V.L. Kosmin; Applicant - Research R.R. Vreden Institute of Traumatology and Orthopedics” of the Ministry of Health of the Russian Federation. (In Russ).
8. Tsumaki N. Cartilage regeneration using cell reprogramming technologies. Clin Calcium. 2013;23(11):1641-1648.
9. Zorina A. I., Zorin V. L., Cherkasov V. R. et al. Cellular technologies in aesthetic medicine. Modern problems of dermatovenereology, immunology and medical cosmetology. 2010;4(4):76-82. (In Russ).
10. Araque-Monrós M.C., García-Cruz D.M., Escobar-Ivirico J.L., Gil-Santos L., Monleón-Pradas M., Más-Estellés J. Regenerative and Resorbable PLA/HA Hybrid Construct for Tendon/Ligament Tissue Engineering. Ann Biomed Eng. 2020. Published online.
Review
For citations:
Bozhokin M.S., Marchenko D.M., Mikhailova E.R., Antipov A.P., Nashchekina Yu.A., Vcherashnij D.B., Novoseltsev S.V., Kruglov V.N. Primary results of replacement the experimental hyaline cartilage defect with human fibroblast cell culture. Bulletin of the Medical Institute "REAVIZ" (REHABILITATION, DOCTOR AND HEALTH). 2022;12(2):14-21. (In Russ.) https://doi.org/10.20340/vmi-rvz.2022.2.MORPH.1
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