The aim of this work is to provide basic answers to questions related to the vaccination of solid organ transplant recipients based on the latest literature sources and clinical guidelines. Vaccination of solid organ transplants and persons in constant contact with them is a mandatory measure, the provision of which is a vital measure that effectively reduces the risk of death from COVID-19. Doubts about less effectiveness in conditions of immunosuppression or the potential risk of developing adverse reactions are significantly inferior to the risk of death and the development of severe complications. Until new data become available, the vaccination regimen must strictly comply with the instructions for medical use of the drug. Before transplantation, the patient should be examined according to the screening protocol, and also consulted by the transplant doctor who supervises him. It is possible that whole virion vaccines (CoviVac) have an advantage over others when vaccinating solid organ recipients, but clinical experience in this matter has not yet been obtained.

The results of this study indicate a lack of adherence to immunoprophylaxis of coronavirus infection both among the surveyed pharmaceutical workers, who are both senior students of the pharmaceutical faculty, and the population, and the need for educational, informational and educational work among them. The topic of immunization of coronavirus infection and other infectious diseases can be one of the topical topics in advanced training courses for pharmacists and pharmacists. In turn, the information and educational work of the pharmacist among the population, carried out in accordance with the labor functions of professional standards, can help improve people's knowledge about the device and mechanisms of action of vaccines against coronavirus. Comprehensive and understandable informing the population about vaccines, from scientific, medical, pharmaceutical, ethical, religious and other positions, can help to overcome internal anti-vaccination attitudes in people and form a commitment to immunoprophylaxis of coronavirus infection and other infectious diseases. In general, this will help people make an informed, independent and free decision about immunization.

Colorectal cancer occupies one of the leading positions in the world in the structure of cancer incidence. The vital processes of cancer cells largely depend on the production of growth factors and their receptors. One of these is epidermal growth factor (EGFR), which is a tyrosine kinase receptor for cell membranes. Normally, binding of EGFR ligands and transforming growth factor alpha (TGFα) induces receptor activation, which triggers ERK and PI3K signaling pathways that control cell proliferation, migration, invasion, and many other processes. It was found that in 80% of cases, colorectal cancer occurs as a result of EGFR overexpression, which leads to increased growth and division of tumor cells due to hyperactivation of the RAS-RAF-MEKERK signaling cascade. The RAS-RAF-MEK-ERK cascade is a pathway that regulates cell proliferation, cell cycle, and cell migration. In the development of human cancer, mutations of the RAS/RAF family are most often the cause of dysregulation of signal transduction through this pathway. According to current data, about a third of all malignant neoplasms are associated with mutations in the genes of the RAS family, which include HRAS, KRAS, NRAS, RRAS, and other homologous proteins. Proteins of the RAS family are involved in the activation of tyrosine kinase signaling pathways, which leads to gene mutations. This process determines proliferative activity, ability to differentiate, metastasis, avoidance of apoptosis, induction of angiogenesis. Permanent RAS activation leads to malignant cell degeneration. Thus, the expression and mutation of the EGFR gene are associated with various variants of tumor progression and poor prognosis in malignant neoplasms of various localizations. Over the past decades, significant advances have been made in the treatment of metastatic colorectal cancer. However, the expansion of the spectrum of effective anticancer drugs also creates a number of difficulties in choosing the optimal drug therapy regimens in patients with metastases of colorectal cancer.

The purpose and objectives of the study: is to create an experimental model of a chemical burn of the esophagus with alkali to study the pathological transformation of the tissues of the upper gastrointestinal tract.

Materials and methods. On the basis of the Department of Experimental Medicine with a vivarium of the Privolzhsky Research Medical University, the study of a chemical burn of the esophagus was carried out on 15 sexually mature male Wistar rats. The burn was simulated by exposure to caustic soda (NaOH) with a concentration of 20 %, 15 %, 10 % and 5 % in a volume of 1 ml. As part of the morphological study, the experimental material was fixed in 10 % formalin. After fixation, the preparations were sent to a standard histological wiring using an Excelsior ES apparatus (Thermo Scientific). Pouring into paraffin blocks using the “HistoStar” filling station (Thermo Scientific), staining with hematoxylin and eosin. For morphometric processing and creation of a video archive of the obtained material, a Leica 2500 microscope, ×4, ×10, ×20, ×40, objective, ×10 eyepiece based on the morphology department of NIITO PIMU was used.

Results. Seeding with a 20 % alkali solution for animals turned out to be incompatible with life, since a day after the injection of the substance, 3 rats died, 2 were in an agonal state. Morphological examination revealed total necrosis (100 %) of the epithelial lining up to the muscle layer and partially in the muscle layer. When burned with a 15 % NaOH solution, the males were in a very serious condition, out of 5 inoculated in 2 cases, a lethal outcome was noted on the 1st day. Histological examination revealed massive necrosis (70 %) of the epithelial lining. The effect of 10 % alkali on the general condition of the animals did not show a pronounced severity of injury. Necrosis was diagnosed on 15 % of the area of the epithelial lining. A burn with 5 % sodium hydroxide solution did not provoke a severity incompatible with life. In this condition, focal necrosis was revealed, occupying about 5 % of the organ area.

Conclusion. Seeds with 10 % and 5 % caustic solutions are relevant for further experimental research, since they make it possible to test different methods of diagnostics, treatment and prevention of scarring at different depths and morphological features of damage.

Obstructive jaundice is a complex pathology that significantly complicates surgical interventions and is accompanied by a number of negative consequences. In connection with the danger of obstructive jaundice, it is very important to timely identify the severity of this condition and take appropriate measures to treat the patient. To date, methods for predicting the risk of postoperative complications in obstructive jaundice are being actively developed, and methods for treating this pathology are being developed. In this study, we studied the biochemical parameters of the functional state of the liver of dogs with simulated obstructive jaundice with different treatment regimens. 3 experimental groups were formed. Control dogs received no treatment. The dogs of the second group underwent decompression of the biliary tract without control of the rate of bile outflow. In animals of the third group, decompression of the biliary tract was carried out and the rate of bile outflow was controlled. As therapy, dogs of this experimental group received Cytoflavin (0.28 ml / kg body weight) and sulodexide (1 ml / kg body weight) under conditions of moderate hypothermia. All animals underwent laparotomy with puncture of the gallbladder and subsequent introduction of a catheter through the cystic duct into the common bile duct. Blood from the portal vein and lymph from the main lymphatic were taken for the analysis of the following biochemical parameters: direct bilirubin, C-reactive protein, alanine aminotransferase, gammaglutamyl transferase and glucose. The concentration of the listed indicators of the functional state of the liver was assessed at different times after the simulation of obstructive jaundice. The results of the study showed that in dogs of all groups, one day after modeling obstructive jaundice, biochemical parameters significantly increase both in the blood of the portal vein and in the lymph of the thoracic lymphatic duct, which is associated with a violation of the functional state of the liver. In animals in which the rate of bile outflow was not controlled, biochemical parameters remained at a high level, which reflected the development of liver failure and reperfusion syndrome. After administration of cytoflavin and sulodexide in moderate hypothermia, dogs showed a decrease in the concentration of biochemical parameters to the control level, which characterizes the normalization of the functional state of the liver. In general, the proposed treatment regimen has shown high efficiency and can be used to correct post-decompression liver dysfunction in dogs with obstructive jaundice. The results of the study are valuable for the development of methods for treating patients with obstructive jaundice and preventing the development of postoperative complications. This article can be informative for specialists in the field of surgery and anesthesiology who are involved in the correction of postoperative complications in patients with obstructive jaundice.

Introduction. Pathology of the inferior turbinates (IT) was found in 76.1% of patients with difficulty in nasal breathing. If nasal breathing cannot be restored with conservative measures, surgical intervention becomes the method of choice.

Materials and methods. Under local infiltration anesthesia, the IT is fractured and retracted medially (medialization). In the anterior section of the HHP, a vertical incision 0.5 cm long to the bone is made, through which the mucosa is peeled off from the bone base of the concha with a raspator-suction and a small tunnel from 2 to 3 cm long is created. shell surfaces and a small area of the mucosa of the lateral surface of the IT and the lateral wall of the nasal cavity. After insertion into the created tunnel of the required size of the "spreader-implant" plate, treated with a thin layer of LTK glue, the shell is slightly pressed with a self-inflating latex-foam rubber swab introduced into the IT for 10–15 minutes. At the end of the operation, after removing the tampon, the edges of the incision are processed with LTK glue.

Results. The results of the study demonstrate the effectiveness of the developed method for the rapid restoration of nasal breathing after rhinosurgical interventions. We use this technique in the overwhelming majority of cases of fibrous and bone forms of IT hypertrophy. In cases of the cavernous form, we perform a submucous conchotomy with a shaver, which makes it possible to very accurately remove exactly that part of the concha that violates the patency of the nasal cavity. In all cases, we consider it compulsory to carry out the IT lateroposition according to our method, which significantly improves the result of the operation. The surface of the shell remains completely covered with mucosa and as a result of matching the edges of the incision, the wound surface is absent. At the same time, after the operation, the shell remains full anatomically and functionally, i.e. consists of the bone skeleton and the corresponding volume of soft tissues.

Conclusion. The question of how to predict in the long-term postoperative period the normal size of the modeled IT, apparently, is currently not answered even by highly qualified rhinosurgeons. In this regard, there remains a need to search for new technologies for treating patients with chronic hypertrophic rhinitis.

Femoral hernias make up 2–4 % of the total number of patients with hernias [1], the results of their treatment do not tend to improve [2–6].

The aim of the study was to establish the incidence of atrophy of the pubic periosteum in patients with femoral hernia and to analyze the possible methods of surgery in their treatment.

Material and methods. The analysis of the treatment of 249 patients with femoral hernias for the period from 1996 to 2021 was carried out. There were 61 men (24.5%), women – 188 (75.5%). in 14 (5.6%), atrophy of the pubic periosteum was revealed during operations. Since 2009, patients with femoral hernia and atrophy of the pubic periosteum began to use the "Method for the treatment of inguinal and femoral hernias" (patent for invention No. 2445002, authors V.I. Belokonev, A.V. Vavilov, A.V. Zharov, Yu. V. Ponomareva, A.G. Nogoga) [11], which was performed by inguinal access.

Conclusions. In 5.6% of patients with femoral hernia and a long history, under the influence of the hernial sac, atrophy of the pubic periosteum occurs.A method of treating femoral and inguinal hernias by closing the hernial orifice with an elastic mesh with a protrusion at the medial edge of the mesh corresponding to the distance from the medial edge of the pupar ligament at the level of the femoral vein to the lower edge of the superior horizontal branch of the pubic bone (patent for invention No. 2445002) is an effective method of treating patients with hernias with destruction of the pubic bone periosteum in the femoral canal.

Aim. Highlighting the immediate and long-term results of using a biocarbon implant in comparison with the standard method of using a polypropylene implant when operating on patients with large and giant diaphragmatic hernias.

Materials and methods. All patients were divided into 2 study groups, which underwent alloplasty with various implants: Group I of 221 patients who underwent alloplasty with a polypropylene mesh implant (171 patients with large hernias with an area of 10–20 cm2 and 50 patients with giant hiatal hernias with the area of the hernial defect is more than 20 cm2); Group II of 79 patients who underwent original alloplasty with a two-layer biocarbon mesh implant (50 patients with large hernias and 29 patients with giant hiatal hernias). Postoperative complications were classified according to the Clavien-Dindo scale. The De Meester Index was used as a comparison criterion.

Results. The results of surgical treatment are pilot and representative, which determine the further tactics and direction of improving operations to remove large and giant diaphragmatic hernias. The data on the use of a two-layer biocarbon implant and a comparison with a polypropylene implant during onlay repair of large and giant diaphragmatic hernias are presented.

Conclusion. There were significant differences in relapses of all types in favor of a biocarbon implant (5,6 versus 22,8%; p < 0,0001; Fisher's exact test).

Gallbladder polyps are an elevation of the mucous membrane that protrudes into the lumen of the gallbladder cavity. Pathology, according to world literature, occurs with a frequency of 0.3% to 13.8%. Currently, the diagnosis of polypoid formations of the gallbladder is based on the routine use of ultrasound, without further clarifying research, which often leads to errors in treatment tactics. In the domestic literature, there are very few studies on this topic, despite the relevance of this area.

Relevance. Prolonged apical stimulation of the right ventricle can lead to the development of interventricular and intraventricular dyssynchrony. Selective (alternative) stimulation of various parts of the interventricular septum due to a more physiological propagation of the excitation wave makes it possible to prevent the deterioration of the mechanical function of the myocardium associated with constant pacing.

Purpose. To study the dynamics of indicators of electrical and mechanical ventricular dyssynchrony in the traditional (apical) and alternative with the installation of a right ventricular electrode in the interventricular septum or the outlet tract of the right ventricle in patients with constant pacing in the early and late postoperative period.

Materials and methods. Were included 73 patients (mean age 61.0 ± 2.5 years) with indications for continuous cardiac pacing without signs of heart failure above 2 FC according to NYHA and previous Q-forming myocardial infarction. Patients of group 1 (n = 39) were implanted with a pacing ventricular electrode in the middle section of the interventricular septum (subgroup 1A, n = 25) or in the outflow tract of the right ventricle (subgroup 1B, n = 14). The control group 2 included 34 patients with electrode implantation in the apex of the right ventricle.

Results. The time of pre-ejection from the right and left ventricles before and after implantation did not differ in group 1 and significantly differed in group 2 (25 ± 3 ms and 50 ± 6 ms, p < 0.05). There was no significant difference in the parameters of ventricular remodeling between the groups within 2 years, but there was a tendency towards a lower value of the left ventricular ejection fraction (59 ± 6 % and 50 ± 2 %, p = 0.05) and a higher frequency of mitral regurgitation 2 degree and higher in patients in the apical stimulation group. Within the group of alternative stimulation, after 2 years of follow-up, there was a tendency towards a smaller value of the delay time of systolic contraction between the basal segments of the free wall of the right ventricle and the interventricular septum during stimulation of the outflow tract compared with stimulation of the middle part of the interventricular septum (30 ± 8 ms and 38 ± 10 ms , p = 0.05).

Conclusions. Alternative stimulation of the right ventricle compared with the apical is characterized by a lower degree of interventricular dyssynchrony both immediately after surgery and in the long-term period. New echocardiographic technologies, including tissue myocardial Doppler ultrasonography, make it possible to quantify the indicators of systolic and diastolic myocardial function and various parameters of dyssynchrony.

Conclusion. To assess the physiology, clinical efficacy and safety of the new method of stimulating the interventricular septum, a more thorough study of the severity of intra- and interventricular dyssynchrony, indicators of global and regional systolic and diastolic function of the left and right ventricles is necessary.

The aim of the study is to improve the quality of life of women with breast cancer (BC) and a high risk of its development by performing one-stage or delayed reconstruction of the lost breast.

The objectives of the study were: to clarify the criteria for selecting patients for prophylactic mastectomy; development of surgical tactics in the treatment of breast cancer and a high risk of its development; assessment of oncological safety of LME; clarification of the methods of reconstruction of the breast; analysis of postoperative complications, including the effect of neoadjuvant PCT during simultaneous breast reconstruction on their frequency, and determination of possible ways to prevent them.

Material and methods. The prospective controlled study included 258 patients who underwent surgical treatment in our department between 2007 and 2016. The criteria for the inclusion of patients in the study were: carrying out radical surgical treatment for breast cancer, both in isolation and in combination with the reconstruction of the lost breast; carriage of germline mutations in the tumor suppressor genes BRCA1 and BRCA2, the presence of first-degree relatives suffering from breast cancer, previous BRCA-associated breast cancer; the desire of patients with multiple recurrent proliferative benign breast diseases that are not amenable to conservative and surgical treatment to use the surgical method of breast cancer prophylaxis with immediate restoration of the breast.

Results. According to the Beck Depression Questionnaire, 72 % of patients in the first group of patients had a critical and high level of depression, while patients who underwent CME with one-stage reconstruction did not have such levels of depression. At the same time, a relatively favorable psychological state of patients with a low level of depression was observed in 17 of 21 patients in the RME + delayed reconstruction group (which amounted to 81% of the group), in 21 of 22 patients in the RME + simultaneous reconstruction group (95% of the group), and only in 5 of 43 patients in the RME group (12% of the group) (χ2 = 51.6; critical value 9.2 at p ≤ 0.01).

Conclusions. When analyzing the results obtained, we once again became convinced of the oncological safety of LME with a one-stage reconstruction of the breast, subject to certain requirements. In the presence of appropriate conditions, it is possible to preserve the SAC during the LME. Preventive LME with simultaneous breast reconstruction is the method of choice in patients with a high risk of developing breast cancer, subject to the appropriate selection criteria and the patient's desire.

Despite the active introduction of surgical treatments for cardiac pathology and new synthetic cardiotonic drugs, cardiac glycosides (CG) remain an important component of the pharmacological treatment of heart diseases. Moreover, interest in this group of drugs is only increasing with the discovery of new pharmacological effects, such as antitumor and antiviral. However, despite many years of experience, the issue of managing patients with symptoms of both acute and chronic poisoning with cardiac glycosides remains one of the leading ones. The literature review provides a brief historical outline of cardiac glycoside poisoning and comprehensively describes the pharmacokinetics and pharmacodynamics of this group of drugs. The clinical picture of intoxication is described. We analyzed modern ideas and limitations of using antidote therapy such as digoxin immune Fab-antibody fragments, as well as the features of pathogenetic and symptomatic treatment of patients with acute glycoside poisoning.

A common mistake of an orthopedic dentist when carrying out odontopreparation of teeth is nonobservance of the parameters of the permissible convergence of the axial walls of the teeth [1]. Analysis of the literature allows us to conclude that with the taper of the tooth walls at 15 degrees, the retention of the crowns is 70 % [2]. The aim of this work was the development of an improved method for coaxial odontopreparation of teeth. This article describes the method of odontopedication of teeth, which are a support for fixed orthopedic structures of an extended nature. The essence of the method is to create a bed on the abutment teeth using a thin fissure bur. The process of coaxial preparation of abutment teeth in the author's technique consists of several stages, which makes it possible to control the preparation accuracy and qualitatively reduce the likelihood of excessive grinding of tooth tissues.

Introduction. The actual problem of dentists-orthopedists at the present time is a violation of the odontopreparation protocol and, as a result, poor-quality fixation of various non-removable orthopedic structures. For a strong and reliable fixation of non-removable orthopedic structures, special requirements must be imposed on them in terms of the shape and surface of the support of the processed tooth. These requirements include: parallelism of the walls of the prepared tooth (must be cylindrical); parallelism of the vertical walls of the stumps of the teeth, high-quality gingival ledge [1, 2]. Qualitatively prepared teeth for orthopedic structures, observance of parallelism of the walls create optimal conditions for stable fixation of orthopedic structures. Correctly executed gingival ledge promotes durable fixation of the crown and eliminates the likelihood of infection under it. After analyzing a large volume of domestic and foreign literature, it can be concluded that the creation of various methods of odontopreparation with the task of creating the most favorable supragingival part of the tooth for further fixation of the orthopedic structure is a significant problem in modern dentistry. The creation and clinical testing of DNT (dental navigation technologies) allows for accurate quality control of the performed odontopreparation. The purpose of this article was to analyze the existing methods of odontopreparation and identify the most effective ones among them.

In memory of academician V.G. Savchenko, who made an invaluable contribution to the development of the program bone marrow transplants both in Russia and in the Samara region.